European Journal of Taxonomy 131: 1-22

http://dx.doi.org/10.5852/ejt.2015.131

This work is licensed under a Creative Commons Attribution 3.0 License.

ISSN 2118-9773

www. europeanj ournaloftaxonomv. eu

2015 • Strandberg J. & Johanson K.A.

Research article

urn:lsid:zoobank.org:pub:DCD171EC-6709-4495-85B3-6430E85C529E

New records of Dasyhelea Kieffer, 1911 from Sweden, with descriptions

of two new species (Diptera: Ceratopogonidae)

Jonas STRANDBERG 1 2 3 * & Kjell Arne JOHANSON 1 4

1 Swedish Museum of Natural History, Department of Zoology, Box 50007,

104 05 Stockholm, Sweden

2 Stockholm University, Department of Zoology, 106 91 Stockholm, Sweden

* Corresponding author: jonas. strandberg@nrm. se

3 urn: lsid: zoobank.org: author:EDE 10C9E-73 7E-4F 88-9FFF-219BDC80F 8B A

4 um:lsid:zoobank.org:author:F2A38CF6-59EB-4F88-BFEB-761DBEA7B01A

Abstract. Based on intensive collecting from various sites in Sweden, the genus Dasyhelea Kieffer, 1911

was reviewed and the number of species now known from the country increased from five to twenty.

Among the new species recorded there are two species described as new to science, D. dominiakae

sp. nov. and D. gothlandica sp. nov., both in the subgenus Dicryptoscena Enderlein, 1936. The following

subgenera are now documented from Sweden: Dasyhelea , Dicryptoscena , Pseudoculicoides Malloch,

1915, Prokempia Kieffer, 1913 and Sebessia Remm, 1979, the two latter subgenera being reported for

the first time.

Keywords. Biting midges, taxonomy, systematics, new species, Malaise trap.

Strandberg J. & Johanson K.A. 2015. New records of Dasyhelea Kieffer, 1911 from Sweden, with descriptions of

two new species (Diptera: Ceratopogonidae). European Journal of Taxonomy 131: 1-22. http://dx.doi.org/10.5852/

eit.2015.131

Introduction

The genus Dasyhelea Kieffer, 1911 is classified in the subfamily Dasyheleinae Lenz, 1934 and is

cosmopolitan in distribution. More than 600 Dasyhelea species are known, representing one-tenth of all

described Ceratopogonidae species in the World (Borkent 2015). The highest diversity is described from

large countries in the northern hemisphere, i.e., China, USA and Russia (Borkent 2015). The immature

stages of Dasyhelea inhabit a wide range of moist and terrestrial habitats, like peat bogs (Domniak &

Szadziewski 2006), rockpools (Wirth 1978), plants of Nepenthes Linnaeus (Wirth & Beaver 1979),

tree sap (Domniak 2005) and in mushrooms (Graves & Graves 1985). The short-lived adults (Zilahi-

Sebess 1931) are often found in or around the vegetation near the breeding sites (Waugh & Wirth 1976).

The structure of the mouthparts in both sexes is similar, i.e., reduction of both mandibles and maxillae

(Glukhova 1981), and both males and females feed on nectar and honey dew and according to Wirth

(1956) they can act as pollinators of plants such as rubber trees ( Hevea brasiliensis (Willd. ex Adr.

Juss.)).

The most recently described ceratopogonid species from Sweden, which are still valid, are Forcipomyia

squamigera Kieffer, 1916 and Bezziapilipennis Lundstrom, 1916. Reports thereafter added more species

European Journal of Taxonomy 131 : 1-22 ( 2015 )

records for the country, resulting in a record list of approximately one hundred species of Ceratopogonidae

for Sweden (Rehnberg & Brodin 2010; Nielsen et al. 2010; Szadziewski et al. 2012). This may be

compared to 184 species in Estonia, 211 in Poland and 256 in Germany (Szadziewski et al. 2012), which

indicates that Sweden might have been undersampled historically compared to some other countries.

Efforts were therefore made in order to more carefully examine the Swedish diversity using recently

trapped material from various sites. The Swedish Dasyhelea include five species previously recorded

from Sweden: D. pallidiventris (Goetghebuer, 1931), D. corinneae Gosseries, 1991, D. turficola Kieffer,

1925, D. modesta (Winnertz, 1852) and D. notata Goetghebuer, 1920 (Dominiak & Szadziewski 2010).

With this report the number of Dasyhelea species in Sweden increases to 20, a low number compared to

the more than 60 species recorded from the rest of Europe (Szadziewski et al. 2012).

Material & Methods

The specimens in this study were collected from Djaknabygds Bokbacke during the summer of 2005,

from Limhmans lcallcbrott (limestone quarry) during the summer of 2009, and various other localities

in Sweden during the spring and summer of 2011. Specimens were collected in Malaise traps and in

sweep nets. At the lab they were stored in 80% ethanol in deep freezers. After sorting and preliminary

identification, the whole body was cleared using Proteinase K as part of DNA extraction and sequenced

for mitochondrial cytochrome I (COI) according to the methods described in Strandberg & Johanson

(2010). The COI sequences were used to associate males and females using MEGA 5.2.2 (Tamura et

al. 2011), applying the settings in Stur & Borkent (2014). The head, wings and abdomen/genitals from

the thorax and one leg from each leg pair were mounted under separate cover slips on microscope

slides using Euparal as permanent mounts. Specimens from Limhamns kalkbrott and Bunkeflostrand are

stored at the Museum of Zoology, Lund University, Sweden (MZLU) while the rest are deposited at the

Department of Zoology, Swedish Museum of Natural History (NHRS).

In the descriptions, specimens were measured for each character and ratios and measurements of the

antennae (AR), palpi (PR (III) ), wings and costal vein (CR) were obtained following the methods described

by Dominiak (2012). Measurements are given for the holotype as well as the range of variation in each

character for the paratypes (n = number of specimens examined).

Results

Class Insecta Linnaeus, 1758

Order Diptera Linnaeus, 1758

Family Ceratopogonidae Newman, 1834

Subfamily Dasyheleinae Lenz, 1934

Genus Dasyhelea Kieffer, 1911

Dasyhelea Kieffer, 1911: 5. Type species: Dasyhelea halophila Kieffer, 1911: 5 (by monotypy).

Subgenus Dasyhelea

Dasyhelea ( Dasyhelea ) bensoni Edwards, 1933

Dasyhelea bensoni Edwards, 1933: 91.

Dasyhelea vernalis Remm, 1979: 56.

New record

SWEDEN: 2 Gallivare, Muddus National Park, Lake Muddus, 67.030916° N, 20.254950° E,

418 masl, loc. 036-01, Malaise trap, 7-20 Jun. 2011, leg. H. Gustafsson (NHRS).

STRANDBERG J. & JOHANSON K.A., New records of Dasyhelea from Sweden

Distribution

Great Britain, Norway, Estonia, Romania, Russia (Amur Oblast, Yakutia) (Dominiak & Szadziewski

2010 ).

Biology

Specimens were collected on mire close to a lake during mid June. Previously D. bensoni has been

collected from the end of May to mid July (Edwards 1933; Remm 1979).

Dasyhelea {Dasyhelea) bilineata Goetghebuer, 1920

Dasyhelea bilineata Goetghebuer, 1920: 45.

Dasyhelea insignipalpis Kieffer, 1925a: 62 (as D. versicolor var. insignipalpis).

Tetraphora saxicola Edwards, 1929: 426.

Dasyhelea geleiana Zilahi-Sebess, 1931: 321.

Dasyhelea montana Zilahi-Sebess, 1940: 48 (asD. dufouri var montana).

Dasyhelea lithotelmatica Strenzke in Thienemann, 1950: 178.

Dasyhelea< tecticolaRemmert, 1953: 334.

Culicoides dieuzeidei Vaillant, 1957: 265.

Dasyhelea karelica Glukhova & Brodskaya, 1997: 443.

New record

SWEDEN: 1 1 §, Mahno, Bunkeflostrand, Malaise trap, 16 Jul.-l Aug. 2009, leg. B.W. Svensson

(MZLU).

Distribution

Norway, Finland, Russia, Estonia, Great Britain, Ireland, Belgium, Germany, Poland, Czech Republic,

Slovakia, France, Switzerland, Austria, Italy, Croatia, Hungary, Romania, Ukraine (Crimea), Bulgaria,

Spain, Gibraltar, Algeria, Turkey (Dominiak & Szadziewski 2010).

Biology

The specimens were collected in an area with shore meadows on the west coast in the south of Sweden.

The larva inhabits shallow water bodies; both natural and artificial and preimaginal stages are very

tolerant of desiccation and temperature fluctuations (Zilahi-Sebess 1931; Valkanov 1941; Disney 1975).

Remarks

The male keys out as D. bilineata in Dominiak (2012). The associated female has the subgenital plate

with broad lateral processes and a more hearth-shaped notum in contrast to the isosceles triangular¬

shaped notum of D. bilineata shown in Dominiak (2012).

Dasyhelea {Dasyhelea) caesia Remm, 1993

Dasyhelea caesia Remm, 1993: 193.

Dasyhelea lugensis Brodskaya, 1995: 9.

New record

SWEDEN: 1 <$, Haninge, Tyresta National Park, Lopantrask, 59.182483° N, 18.303100° E, 40 masl,

loc. 025-07, Malaise trap, 19 Jul.-2 Aug. 2011, leg. J. Strandberg (NHRS).

European Journal of Taxonomy 131 : 1-22 ( 2015 )

Distribution

Russia (Leningrad Oblast, Yakutia), Poland (Dominiak & Szadziewski 2010).

Biology

The specimen was collected in a marsh situated between two lakes with water flowing through. Last

instar larvae and pupae of Dasyhelea caesia have previously been collected from a small body of water

and the littoral zone of lakes in Russia during July (Brodskaya 1995; Przhiboro 2005).

Dasyhelea ( Dasyhelea ) malleola Remm, 1962

Dasyhelea malleolus Remm, 1962: 119.

New records

SWEDEN: 2 SS, 1 $, Gallivare, Muddus National Park, Lake Muddus, 67.030916° N, 20.254950° E,

418 masl, loc. 036-01, Malaise trap, 7-20 Jun. 2011, leg. H. Gustafsson (NHRS); 1 3 $ $, Gallivare,

Muddus National Park, Lake Muddus, 67.030916° N, 20.254950° E, 418 masl, loc. 036-02, Malaise trap,

20 Jun -3 Jul. 2011, leg. H. Gustafsson (NHRS); 2 SS, Gallivare, Muddus National Park, 67.049916° N,

20.256533° E, 443 masl, loc. 037-01, Malaise trap, 7-20 Jun. 2011, leg. H. Gustafsson (NHRS); 1 §,

Gallivare, Muddus National Park, 67.049916° N, 20.256533° E, 443 masl, loc. 037-02, Malaise trap,

20 Jun-3 Jul. 2011, leg. H. Gustafsson (NHRS).

Distribution

Estonia, Norway, Germany, Poland, Czech Republic, Ukraine (Crimea), Spain, Andorra, Algeria

(Dominiak & Szadziewski 2010; Stur & Borkent 2014).

Biology

Specimens were collected on mire next to a lake from the beginning of June to the beginning of July.

Dasyhelea (Dasyhelea) jiavifrons (Guerin, 1833)

Ceratopogon flavifrons Guerin, 1833: 165.

Ceratopogon oh scums Winnertz, 1852: 45.

Ceratopogon versicolor Winnertz, 1852: 45.

Ceratopogon dufouri Laboulbene, 1869: 158.

Ceratopogon hippocastani Mile, 1888: 185.

Ceratopogon rufithorax Strobl, 1920: 261 (as C. versicolor x ar. rufithorax).

Dasyhelea brevitibialis Goetghebuer, 1919: 72.

Dasyhelea goetghebueri Kieffer, 1919: 53.

Dasyhelea sensual is Kieffer, 1919: 55.

Dasyhelea lignicola Kieffer, 1919: 57.

Dasyhelea paludicola Kieffer, 1925b: 152.

Dasyhelea opressa Thomsen, 1935: 285.

Dasyhelea septuosa Borkent in Borkent & Wirth, 1997: 58 (new name for D. obscura (Winnertz, 1852)).

New record

SWEDEN: 1 Nybro, Alsterbro, Alsteran, 56.936536° N, 15.920167° E, Trap ID 1008, collecting

event 1743, Malaise trap, 16-22 Jul. 2006, leg. Swedish Malaise Trap Project (NHRS).

STRANDBERG J. & JOHANSON K.A., New records of Dasyhelea from Sweden

Distribution

Estonia, Great Britain, Belgium, Germany, Poland, Czech Republic, France, Austria, Switzerland,

Spain, Croatia (Istria), Greece (Crete), Bulgaria, Ukraine (Crimea), Russia (Karachay-Cherkessia), USA

(Dominiak & Szadziewski 2010).

Biology

Larvae of D. flavifrons can be found in tree sap or tree holes. Flight period May-October (Dominiak

2012 ).

Remarks

The previous listing of this species as present in Sweden is doubtful as it has only been reported in

Rehnberg & Brodin (2010), based on D. versicolor (Winnertz, 1852) as listed in Szadziewski et al.

(1997). We were unable to verily previous records.

Dasyhelea ( Dasyhelea ) pallidiventris (Goetghebuer, 1931)

Tetraphora pallidiventris Goetghebuer, 1931: 211.

Dasyhelea olivacea Remm, 1962: 117.

Distribution

Sweden, Finland, Estonia, Lithuania, Germany, Poland, Czech Republic, Ukraine (Crimea), Georgia,

Azerbaijan, North Korea (Dominiak & Szadziewski 2010).

Biology

Dasyhelea pallidiventris is considered a haloxene, and the larvae inhabit inland saline habitats and the

littoral zone of lakes (Szadziewski 1983). Previously recorded from Norrtalje in east-central Sweden

(Dominiak & Szadziewski 2010).

Subgenus Prokempia Kieffer, 1913

Prokempia Kieffer , 1913a: 163, 179. Type species: Dasyhelea ornaticornis Kieffer, 1913a: 180 (desig¬

nated by Wirth 1973: 358).

Dasyhelea ( Prokempia ) biunguis Kieffer, 1925

Dasyhelea biunguis Kieffer 1925c: 409.

New record

SWEDEN: 1 1 §, Almhult, Stenbrohult, Djaknabygds bokbacke, 56.609133° N, 14.193050° E, Trap

ID 24, collecting event 1673, Malaise trap, 31 May-20 Jul. 2005, leg. Swedish Malaise Trap Project

(NHRS).

Distribution

Great Britain, Norway, Estonia, Russia (Kaliningrad Oblast) (Dominiak & Szadziewski 2010).

Biology

Specimens collected on a heath with old beeches.

European Journal of Taxonomy 131: 1-22 (2015)

Dasyhelect ( Prokempia ) dampfi Kieffer, 1925

Dasyhelea dampfi Kieffer, 1925b: 150.

Dasyhelea turfacea Kieffer, 1925b: 151.

Dasyhelea estonica Kieffer, 1925c: 409.

New record

SWEDEN: 1 Gnosjo, Store mosse, Kittlakull, 57.287233° N, 13.919133° E, Trap ID 41, collecting

event 1159, Malaise trap, 3-6 Aug. 2004, leg. Swedish Malaise Trap Project (NHRS).

Distribution

Estonia, Lithuania, Poland, Czech Republic (Dominiak & Szadziewski 2010).

Biology

This specimen was collected at a peat bog in the beginning of August.

Subgenus Pseudoculicoides Malloch, 1915

Pseudoculicoides Malloch, 1915: 309. Type species: Ceratopogon mutabilis Coquilett, 1901: 602 (by

orginal designation).

Dasyhelea (Pseudoculicoides) arenivaga Macfie, 1943

Dasyhelea inconspicuosa \ ar. arenivaga Macfie, 1943: 151.

New records

SWEDEN: 1 S, 1 Gotland, 57.847400° N, 18.811216° E, 3 Jul. 2011, 6 masl, net, leg. J. Strandberg

(NHRS); 1 S, 3 ??, Gotland, Bastetrask, 57.899383° N, 18.901083° E, 9 masl, loc. 027-04, Malaise

trap, 28 May-12 Jun. 2011, leg. B. Eklund (NHRS).

Distribution

Poland, Czech Republic, Switzerland, Romania, Ukraine (Crimea), Bulgaria, Spain, Algeria, Egypt,

Israel, United Arab Emirates (Dominiak & Szadziewski 2010; Szadziewski et al. 2011).

Biology

These specimens were collected in a marsh and in a seasonally flooded area next to a lake. D. arenivaga

is known to visit umbelliferous and tamarisk flowers (Dominiak & Szadziewski 2010).

Dasyhelea (Pseudoculicoides ) calycata Remm, 1972

Dasyhelea calycata Remm, 1972: 74.

New record

SWEDEN: 1 1 ?, Torsas, Soderakra, Paboda, 56.434723° N, 16.070844° E, Trap ID 2046, collecting

event 2058, Malaise trap, 1 Sep.-3 Oct. 2008, leg. Swedish Malaise Trap Project (NHRS).

Distribution

Great Britain, Lithuania, Poland, Slovakia, Bosnia and Herzegovina, Ukraine (Crimea), Romania,

Bulgaria, Spain, Hungary, Russia (North Ossetia, Tuva, Buryatia), Mongolia (Dominiak & Szadziewski

2010 ).

STRANDBERG J. & JOHANSON K.A., New records of Dasyhelea from Sweden

Biology

The specimens were collected in a small village garden. Larvae can be found in both fresh and salt-water

habitats and are co mm on on inland saline meadows in Poland (Dominiak 2012).

Remarks

The association of the sexes made by Remm (1972) has previously been considered incorrect. The

associated female probably belongs to D. unguistyla Remm, 1972 (Dominiak & Szadziewski 2010). The

female associated in this study by COI sequences differs from the original description in lacking long

cerci and in having a subgenital plate with a lumen. According to Dominiak & Szadziewski (2010) the

type material is unavailable.

Dasyhelea ( Pseudoculicoides ) corinneae Gosseries, 1991

Ceratopogon scutellatus MQig&n, 1830: 262.

Dasyhelea corinneae Gosseries, 1991: 42 (new name forD. scutellatus Meigen, 1830).

Dasyhelea chonetus Yu & Zou in Yu et al., 2006: 279.

Distribution

Great Britain, Sweden, Poland, Hungary, Ukraine, Russia (North Ossestia, Ussuri Land), China, North

Korea, Canada, USA (Dominiak & Szadziewski 2010).

Biology

The only specimen recorded from Sweden was collected at Antjarn in August (Dominiak & Szadziewski

2010 ).

Dasyhelea ( Pseudoculicoides ) europaea Remm, 1962

Dasyhelea europaea Remm, 1962: 122.

New records

SWEDEN: 1 Gallivare, Muddus National Park, 67.049916° N, 20.256533° E, 443 masl, loc. 037-

01, Malaise trap, 7-20 Jun. 2011, leg. H. Gustafsson (NHRS); 1 S, Gotland, Larbro, 57.847400° N,

18.811216° E, 6 masl, net, 3 Jul. 2011, leg. J. Strandberg (NHRS).

Distribution

Norway, Estonia, France, Czech Republic (Dominiak & Szadziewski 2010).

Biology

Specimens collected on mire in the north of Sweden and from the vegetation in a marsh in the southeast

of Sweden. It is reported as a spring-summer species, most common between May and June (Remm

1962).

Dasyhelea ( Pseudoculicoides ) turficola Kieffer, 1925

Dasyhelea turficola Kieffer, 1925a: 15.

Dasyhelea grenieri Clastrier, 1966: 703.

Dasyhelea malibui Yu, 2008: 165.

European Journal of Taxonomy 131: 1-22 (2015)

Distribution

Great Britain, Ireland, Sweden, Estonia, Lithuania, Russia (Kaliningrad Oblast, Ussuri Land), Poland,

Czech Republic, Belgium, Prance, Spain (Iberian Peninsula, Canaries: Tenerife), Georgia, Morocco,

Algeria, Israel (Dominiak & Szadziewski 2010).

Biology

Dasyhelea turficola larvae are known to inhabit peat bogs and moist soil close to fresh and salt-water

bodies. Adults of the species visit umbelliferous flowers (Dominiak 2012). Previously, D. turficola have

been collected in Johannisfors (Dominiak & Szadziewski 2010).

Subgenus Dicryptoscena Enderlein, 1936

Dicryptoscena Enderlein, 1936: 51. Type species Dasyhelea inclusa Kieffer, 1918: 188 (by original

designation).

Dasyhelea (Dicryptoscena) modesta (Winnertz, 1852)

Ceratopogon modestus Winnertz, 1852: 43.

Ceratopogon aestivus Winnertz, 1852: 42.

Dasyhelea longipalpis Kieffer, 1913b: 37.

Dasyhelea inclusa Kieffer, 1918: 188.

Dasyhelea strobli Kieffer, 1919: 63.

Dasyhelea pratensis Goetghebuer, 1920: 44.

Dasyhelea bihamata Kieffer, 1923: 667.

Dasyhelea moascari Macfie, 1943: 153.

Dasyhelea densipilosa Tokunaga, 1963: 41.

Distribution

Norway, Sweden, Russia (Karelia, Leningrad Oblast, North Ossetia), Estonia, Lithuania, Great Britain,

Poland, Germany, the Netherlands, Belgium, Czech Republic, France, Switzerland, Austria, Hungary,

Romania, Ukraine (Crimea), Bulgaria, Andorra, Spain, Georgia, Azerbaijan, Afghanistan, Iran, China,

Japan, Algeria, Egypt, Yemen (Dominiak & Szadziewski 2010).

Biology

Preimaginal stages inhabit a wide range of aquatic and semiaquatic habitats and can be found in ponds,

mud, swamps, peat bogs, the littoral zone of lakes and saline habitats (Thienemann 1915, 1950; Mayer

1934; Szadziewski 1986; Przhiboro 1999; Chandler etal. 2008).

Dasyhelea (Dicryptoscena) notata Goetghebuer, 1920

Dasyhelea notata Goetghebuer, 1920: 47.

Dasyhelea semistriata Goetghebuer, 1921: 176.

Dasyhelea sziladyi Zilahi-Sebess, 1936: 42.

Distribution

Sweden, Finland, Estonia, Lithuania, Poland, Belgium, France, Switzerland, Czech Republic, Hungary,

Romania, Spain, Georgia, Russia (Ussuri Land), North Korea, Algeria (Dominiak & Szadziewski 2010).

STRANDBERG J. & JOHANSON K.A., New records of Dasyhelea from Sweden

Biology

Adults of this species are known to visit umbelliferous flowers (Dominiak 2012).

Dasyhelea ( Diryptoscena ) stellata Remm, 1968

Dasyhelea stellata Remm in Remm & Zhogolev, 1968: 831.

New record

SWEDEN: 2 SS, Malmo, Limhamns kalkbrott, 2-“planen”, Malaise trap, 11-25 Jun. 2009, leg. B.W.

Svensson & Co. (MZLU).

Distribution

Great Britain, Poland, France, Czech Republic, Ukraine (Crimea), Russia (North Ossetia) (Dominiak &

Szadziewski 2010).

Biology

These specimens were collected close to a pond in an old limestone quarry in the south of Sweden.

Dasyhelea {Dicryotoscena) thienemanni Spataru & Damian-Georgescu, 1970

Dasyhelea thienemanni Spataru & Damian-Georgescu, 1970: 425.

New records

SWEDEN: 2 $$, Norrtalje, Svartsjon, 60.123098° N, 18.615078° E, 2 masl, loc. 020-01, Malaise trap,

30 Jul.-26 Aug. 2010, leg. J. Strandberg (NHRS); 2 Malmo, Limhamns kalkbrott, l-“grafitti”,

Malaise trap, 26 Jun.-8 Jul. 2009, leg. B.W. Svensson & Co. (MZLU).

Distribution

Estonia, Lithuania, Poland, Slovakia, Hungary, Romania, Bulgaria, Georgia, Azerbaijan (Dominiak &

Szadziewski 2010).

Biology

The specimens were collected from a small peat-bog in the middle-east of Sweden and from an old

limestone quarry in the south of Sweden. Preimaginal stages have previously been found in and around

a spring (Spataru & Damian-Georgescu 1970).

Dasyhelea {Dicryptoscena) dominiakae sp. nov.

um:lsid:zoobank.org:act:956852B2-A7AD-46A6-A238-0F8B55540915

Figs 1-11

Diagnosis

The male resembles those of D. notata in the morphology of the genitalia, particularly by the presence

of a pair of horn-like projections on the posterior margin of sternite 9. It is distinguished from D. notata

in having an additional triangular projection on each lateral side of the posterior margin of sternite 9.

Etymology

This species is named after Dr. Patrycja Dominiak for her contributions to our knowledge of European

Ceratopogonidae, notably the Dasyhelea.

European Journal of Taxonomy 131: 1-22 (2015)

Figs 1-7. Dasyhelea dominiakae sp. nov., holotype, f>. 1. Head. 2. Distal flagellomeres. 3. Frontal sclerite.

4. Clypeus. 5. Palp. 6. Wing. 7. First and second radial cell of wing. Scale bars: 1,6= 100 pm; 3—4,

7 = 50 pm; 2, 5 = 25 pm.

STRANDBERG J. & JOHANSON K.A., New records of Dasyhelea from Sweden

Type material

Holotype

SWEDEN: Slcane, Malmo, Limhamns kalkbrott, 2-“planen”, Malaise trap, 11-25 Jun. 2009, leg.

B.W. Svensson & Co. (MZLU) (DNA voucher: 4DT).

Paratypes

SWEDEN: 3 SS, same data as holotype; 1 S, Skane, Malmo, Limhamns kalkbrott, 1-“graffiti”. Malaise

trap, 28 May-10 Jun. 2009, leg. B.W. Svensson & Co. (MZLU).

Description

Male

Head (Figs 1-5). Ante nn al flagellum length 717 pm (697-747 pm, n = 4); AR 1.15 (1.0-1.28, n = 4)

(Fig. 2). Frontal sclerite rhomboid (Fig. 3). Clypeus entire, with 12 (11-12, n = 4) long setae (Fig. 4).

Palp segment 3 slender, 95 pm (95 pm, n = 4); PR (m) 5.4 (4.75-6.3, n = 4); first half with hyaline

sensillae on inner surface (Fig. 5).

Figs 8-11. Dasyhelea dominiakae sp. nov., holotype, $ . 8-9. Ventral aspect of genitalia. 10. Parameres.

11. Aedeagus. Scale bars: 8-9 = 50 pm; 10-11 = 16 pm.

European Journal of Taxonomy 131: 1-22 (2015)

Thorax (Figs 6-7). Scutellum pale, with 12 bristles (8—11, n = 4). Wing length 1.15 mm (1.02-1.17 mm,

n = 4); CR 0.45 (0.46-0.52, n = 4); macrotrichia numerous (Fig. 6); first radial cell slit-like, second cell

open (Fig. 7). Legs brown, except tarsi pale brown.

Genitalia (Figs 8-11). Apicolateral process of tergite 9 short, with five short bristles. Posterior margin of

sternite 9 with triangular projection on each lateral side and a pair of horn-like projections. Gonostylus

straight, basally broad, apically slender, proximal part covered by small setae, two long bristles at

midpoint present (Figs 8-9). Parameres asymmetrical. Posterior process of parameres fused to right arm,

tapering evenly along its length, evenly curved dorsad (Fig. 10). Aedeagus high arched, with apicolateral

processes twisted along its length, tapering apically (Fig. 11).

Female

Unknown.

Remarks

Dasyhelea dominiakae sp. nov. is a member of the subgenus Dicryptoscena and is similar to D. notata

and D. modesta. What distinguishes this species from those mentioned above, as well as other European

species of Dicryptoscena , is the presence of triangular projections located on sternite 9. The male of D.

dominiakae sp. nov. further differs from D. notata in having an evenly shaped posterior process and it is

also separated from D. modesta by the equal length of the fourth and fifth palpal segments.

Biology

The holotype and paratypes were collected with Malaise traps in an old limestone quarry in the south of

Sweden during May-June, among Salix caprea Linnaeus next to a pond.

Dasyhelea {Dicryptoscena) gothlandica sp. nov.

urn:lsid:zoobank.org:act:5FCC2057-325D-4EFF-B0F9-0C660A747422

Figs 12-32

Diagnosis

The male genitalia of this species are characteristic in having the posterior margin of sternite 9 with a

pair of processes apically separated by a shallow furrow. Gonostyli short, tapering to an indented sharp

point. The aedeagus has broad apicolateral processes, and the posterior process of the paramere has fine

hairs apically.

Etymology

Gothlandica refers to Gotland, a calcareous island located in the Baltic Sea east of mainland Sweden,

where the holotype was collected.

Type material

Holotype

SWEDEN: S, Gotland, Bastetrask, 57.899383° N, 18.901083° E, 9 masl, Malaise trap, 28 May-12 Jun.

2011, leg. B. Eklund (NHRS) (DNA voucher: 4BA).

Paratypes

SWEDEN: 2 2 §?, same data as holotype; 1 Gotland, Horsan, 57.865883° N, 18.846350° E,

4 masl, Malaise trap, 10-25 Jul. 2011, leg. B. Eklund (NHRS); 1 2 §?, Gotland, Bastetrask,

57.899383° N, 18.901083° E, 9 masl, sweep net, 3 Jul. 2011, leg. J. Strandberg (NHRS) (DNA voucher:

6 AW).

STRANDBERG J. & JOHANSON K.A., New records of Dasyhelea from Sweden

Figs 12-18. Dasyhelea gothlandica sp. nov., holotype, <$■ 12. Head. 13. Distal flagellomeres. 14. Frontal

sclerite. 15. Clypeus. 16. Palp. 17. Wing. 18. First and second radial cell of wing. Scale bars: 12, 17 =

100 pm; 14, 18 = 50 pm; 13, 15-16 = 25 pm.

European Journal of Taxonomy 131: 1-22 (2015)

Description

Male

Head (Figs 12-16). Ante nn al flagellum length 727 pm (687-727 pm, n = 4); AR 1.06 (0.97-1.19, n = 4)

(Fig. 13). Frontal sclerite elliptical (Fig. 14). Clypeus entire, with 8 (6-8, n = 4) long setae (Fig. 15).

Palp segment 3 slender, 90 pm (85-91 pm, n = 4); PR 6.6 (6.3, n = 4) (Fig. 16); first half with hyaline

sensillae on inner surface.

Thorax (Figs 17-18). Scutellum pale, with 10 bristles (9-10, n = 4). Wing length 1.15 (1.08-1.17 mm,

n = 4); CR 0.45 (0.46-048, n = 4); macrotrichia numerous (Fig. 17); first radial cell slit-like, second cell

open (Fig. 18). Legs light brown, tarsi paler.

Genitalia (Figs 19-22). Apicolateral process of tergite 9 short. Posterior margin of sternite 9 apically

divided into pair of obtuse projections. Gonostylus short, tapering to indented sharp point, covered

by small setae, two long bristles at midpoint present (Figs 19-20). Parameres asymmetrical; posterior

process fused with right arm; long and slender, distinctly bent apically, with fine apical hairs (Fig. 21).

Aedeagus low arched with apicolateral processes broad, somewhat folded, extended laterally in distal

part (Fig. 22).

Figs 19-22. Dasyheleagothlandicasp. nov., holotype, f. 19-20. Ventral aspect of genitalia. 21. Parameres.

22. Aedeagus. Scale bars: 19-20 = 50 pm; 21-22 =16 pm.

STRANDBERG J. & JOHANSON K.A., New records of Dasyhelea from Sweden

Figs23-29. Dasyheleagothlandica sp. nov., paratype, $. 23. Head. 24. Flagellomeres. 25. Frontal sclerite.

26. Clypeus. 27. Palp. 28. Wing. 29. First and second radial cell of wing. Scale bars: 23, 28 = 100 pm;

25-26, 29 = 50 pm; 24, 27 = 25 pm.

European Journal of Taxonomy 131: 1-22 (2015)

Female

Head (Figs 23-27). Antennal flagellum length 545 jam (444-525, n = 3); distal flagellomeres elongate;

AR 0.96 (0.86-1.0, n = 3) (Fig. 24). Clypeus entire, with 8 (n = 3) long setae (Fig. 25). Frontal sclerite

elliptical (Fig. 26). Palp segment 3 slender, 80 pm (80 pm, n = 4); PR 5.6 (5.3, n = 3) (Fig. 27); with

hyaline sensillae at basal part of inner surface.

Thorax (Figs 28-29). Scutellum pale, with 8 bristles (9-10, n = 3). Wing length 1.1 mm (1.05-1.13,

n = 3); CR 0.46 (0.44-0.47, n = 3) (Fig. 28). First radial cell slit-like, second cell open but small

(Fig. 29). Legs as in male.

Genitalia (Figs 30-32). Subgenital plate with notum separated from ramus, slightly fragmented

(Figs 30-31). Spermatheca single; ovoid-shaped with short neck; length x width: 60 x 40 pm (60 x 40 pm,

n = 3) (Fig. 32).

Remarks

This species is a member of the subgenus Dicryptoscena , and the male is similar to that of Dasyhelea

albidipes Santos Abreu, 1918 in the morphology of the genitalia, particularly by the presence of a pair

of processes on the posterior margin of sternite 9. D. gothlandica sp. nov. differs from D. albidipes by

the broadly shaped apicolateral processes and the short gonostyli.

Biology

The holotype was collected in a Malaise trap next to Lake Bastetraslc, with sourrounding vegetation

consisting of Pinus sylvestris Linnaeus and small shrubs. One of the male paratypes was collected in a

Malaise trap next to Lake Horsan, approximatley 5 km south-east of Bastetrask.

Figs 30-32. Dasyhelea gothlandica sp. nov., paratype, §. 30-31. Subgenital plate. 32. Seminal capsule.

Scale bars = 50 pm.

STRANDBERG J. & JOHANSON K.A., New records of Dasyhelea from Sweden

Subgenus Sebessia Remm, 1979

Sebessia Remm, 1979: 55. Type species: Dasyhelea flavopyga Zilahi-Sebess, 1940: 49 (by original

designation).

Dasyhelea (Sebessia) acuminata Kieffer, 1919

Dasyhelea acuminata Kieffer, 1919: 60.

Dasyhelea polita Edwards, 1921: 124.

Dasyhelea verticillata Kieffer, 1925a: 63.

Dasyhelea littoralis Goetghebuer, 1934: 289.

New records

SWEDEN: 1 S, Morbylanga, Frosslunda alvar, northeastern part, 56.547450° N, 16.577250° E, Trap ID

20, collecting event 1498, Malaise trap, 5 Jul.-2 Aug. 2005, leg. Swedish Malaise Trap Project (NHRS);

3 55, Gotland, Larbro, 57.847400° N, 18.811216° E, 6 masl, net, 3 Jul. 2011, leg. J. Strandberg (NHRS).

Distribution

Great Britain (Scotland), Estonia, Belgium, France, Czech Republic, Hungary, Bulgaria (Dominiak &

Szadziewski 2010).

Biology

The male was collected next to a pasture and the females from the vegetation in a marsh.

Discussion

The two new species belong to the subgenus Dicryptoscena and together with the new records the

Swedish Dasyhelea fauna now contains 20 species, representing the five subgenera, i.e., Dasyhelea (6

spp.), Dicryptoscena (5 spp.), Prokempia (2 spp.), Pseudoculicoides (6 spp.) and Sebessia (1 sp.). The

number of Dasyhelea species in neighboring countries ranges from 8 for Finland (Hulden & Hulden

2014), to 11 for both Norway and Germany (Stur & Borkent 2014; Szadziewski et al. 2012), 12 for

Lithuania, and 30 in both Estonia and Poland (Szadziewski et al. 2012). According to Peterson & Achim

(2001) there are 4 species likely to occur in Denmark based on distributional patterns, and Remm (1988),

without locality data, lists Dasyhelea dampfi as having a distribution that includes Latvia. Comparing

the species composition between neighbouring countries, there are now six species present in Sweden

that have also been reported from either Norway or Finland. The species D. bensoni , D. bilineata and

D. modesta are present in all three countries. Sweden shares 13 species with Estonia and Poland of which

8 are present in all three countries (Szadziewski et al. 2012). Dasyhelea flavoscutellata (Zetterstedt,

1850) has not been recorded from Sweden but is present in all the countries mentioned above except

Lithuania, Denmark and Latvia, and is therefore likely to live in Sweden as well. Most of the new

records for Sweden have been collected from the southern parts of the country and only two records

are from a northern locality (Muddus National Park). The diversity from a major part of Sweden is

thus poorly known and could probably yield many more species. The substantial increase of Dasyhelea

species in Sweden probably signals that the knowledge of Dasyhelea in particular and Ceratopogonidae

in general is poor.

Acknowledgements

The authors are grateful for the help in identifications provided by Dr. Patrycj a Dominiak and Dr. Ryszard

Szadziewski at the Department of Invertebrate Zoology and Parasitology, University of Gdansk, Poland.

For making material available for this study we are particularly indebted to Kajsa Glemhorn, Station

European Journal of Taxonomy 131: 1-22 (2015)

Linne, Oland, Sweden and the staff of the Museum of Zoology in Lund, Sweden. We are very thankful to

Mr. Bertil Eklund, Gotland, Sweden and Mr. Hans Gustafsson, Gallivare, Sweden for emptying Malaise

traps. We are much obliged for the assistance in the lab provided by Dr. Tobias Malm (NHRS). This

study was funded by The Swedish Taxonomy Initiative (dha 157/09 1.4).

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Zilahi-Sebess G. 1940. Heleiden aus Ungarn. Folia Entomologica Hungarica 5: 10-133.

Manuscript received: 25 February 2015

Manuscript accepted: 1 June 2015

Published on: 30 July 2015

Topic editor: Koen Martens

Desk editor: Danny Eibye-Jacobsen

Printed versions of all papers are also deposited in the libraries of the institutes that are members of

the EJT consortium: Museum National d’Histoire Naturelle, Paris, France; Botanic Garden Meise,

Belgium; Royal Museum for Central Africa, Tervuren, Belgium; Natural History Museum, London,

United Kingdom; Royal Belgian Institute of Natural Sciences, Brussels, Belgium; Natural History

Museum of Denmark, Copenhagen, Denmark.